1F5Z
CRYSTAL STRUCTURE ANALYSIS OF N-ACETYLNEURAMINATE LYASE FROM HAEMOPHILUS INFLUENZAE: CRYSTAL FORM I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-11 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.753, 109.778, 133.227 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.880 |
| Rwork | 0.198 |
| R-free | 0.24600 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.060 | 0.400 |
| Total number of observations | 208047 * | |
| Number of reflections | 75195 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 74.0 | 45 |
| Redundancy | 2.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.5 | 293 | Tris-HCl, PEG 4000, NaOAc, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 6 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 20 (mM) | |
| 3 | 1 | 1 | 90 (mM) | ||
| 4 | 1 | 1 | PEG4000 | 30 (%(w/v)) | precipitant |
| 5 | 1 | 1 | Tris-HCl | 0.1 (M) | precipitant |
| 6 | 1 | 1 | 0.1 (M) | precipitant |
