1F5A

CRYSTAL STRUCTURE OF MAMMALIAN POLY(A) POLYMERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-10-08
Detector	BRANDEIS
Spacegroup name	P 21 21 21
Unit cell lengths	57.550, 62.720, 179.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.22 *
Rwork	0.219
R-free	0.26100
RMSD bond length	0.007
RMSD bond angle	22.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.670
High resolution limit [Å]	2.500	2.500
Rmerge	0.085	0.267
Number of reflections	19890
<I/σ(I)>	16.7
Completeness [%]	87.0	65.1
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	24 *	used microseeding *
1	Vapor diffusion *	6	24 *	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	22 (%(w/v))
2	1	reservoir	MES	100 (mM)
3	1	reservoir	ammonium sulfate	120 (mM)
4	1	reservoir		5 (mM)
5	1	reservoir		2 (mM)
6	1	reservoir	beta-mercaptoethanol	2 (mM)