1F45
HUMAN INTERLEUKIN-12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-01 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 112.900, 154.200, 101.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.800 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.28400 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.066 | 0.348 |
| Number of reflections | 21109 | |
| <I/σ(I)> | 25 | |
| Completeness [%] | 96.0 | 96 |
| Redundancy | 5.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 18 * | 12% MPD, 650mM NaCl, 100mM Na Acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | reservoir | MPD | 12 (%(v/v)) | |
| 5 | 1 | reservoir | 550 (mM) | ||
| 6 | 1 | reservoir | sodium acetate | 100 (mM) |
