1F3T
CRYSTAL STRUCTURE OF TRYPANOSOMA BRUCEI ORNITHINE DECARBOXYLASE (ODC) COMPLEXED WITH PUTRESCINE, ODC'S REACTION PRODUCT.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 123 |
Detector technology | CCD |
Collection date | 1999-11-23 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.294, 151.599, 86.414 |
Unit cell angles | 90.00, 103.18, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
R-factor | 0.237 * |
Rwork | 0.237 |
R-free | 0.28000 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 8.000 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 * | 0.319 |
Total number of observations | 402763 * | |
Number of reflections | 111865 * | |
<I/σ(I)> | 19.9 | |
Completeness [%] | 99.3 * | 96.8 |
Redundancy | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 16 * | used microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
10 | 1 | reservoir | HEPES | 100 (mM) | |
11 | 1 | reservoir | dithiothreitol | 10 (mM) | |
2 | 1 | drop | HEPES | 10 (mM) | |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | drop | dithiothreitol | 10 (mM) | |
5 | 1 | drop | EDTA | 0.5 (mM) | |
6 | 1 | drop | Brij-20 | 0.01 (%) | |
7 | 1 | drop | D-Orn | 15 (mM) | |
8 | 1 | reservoir | PEG3350 | 20 (%) | |
9 | 1 | reservoir | 0.2 (M) |