1F3H
X-RAY CRYSTAL STRUCTURE OF THE HUMAN ANTI-APOPTOTIC PROTEIN SURVIVIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-14 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.040, 71.450, 86.630 |
Unit cell angles | 90.00, 133.37, 90.00 |
Refinement procedure
Resolution | 54.120 - 2.580 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.28700 |
RMSD bond length | 0.025 |
RMSD bond angle | 2.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.120 | 2.640 |
High resolution limit [Å] | 2.580 | 2.580 |
Rmerge | 0.036 * | 0.349 * |
Total number of observations | 29469 * | |
Number of reflections | 15366 * | |
Completeness [%] | 95.5 * | 92 * |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | PEG 8000, LITHIUMSULPHATE, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | PEG 8000, LITHIUMSULPHATE, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | PEG 8000, LITHIUMSULPHATE, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | sodium-HEPES | 100 (mM) | pH7.5 |
3 | 1 | reservoir | PEG8000 | 6-8 (%) | |
4 | 1 | reservoir | 200 (mM) | ||
5 | 1 | reservoir | dithiothreitol | 2 (mM) |