1F3G

THREE-DIMENSIONAL STRUCTURE OF THE ESCHERICHIA COLI PHOSPHOCARRIER PROTEIN III GLC

Experimental procedure

Spacegroup name	H 3
Unit cell lengths	80.300, 80.300, 86.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 * - 2.100
R-factor	0.162
RMSD bond length	0.020
RMSD bond angle	3.200
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.100 *
Rmerge	0.021 *
Total number of observations	15038 *
Number of reflections	8279 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	drop	Tris-HCl	25 (mM)
3	1	drop	PEG8000	20 (%(w/w))	precipitant
4	1	drop	sodium acetate	400 (mM)	precipitant
5	1	drop	cacodylate	100 (mM)	precipitant
6	1	reservoir	precipitant		1ml