1F33
THE STRUCTURAL BASIS FOR DNA PROTECTION BY E. COLI DPS PROTEIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 105 |
Detector technology | IMAGE PLATE |
Collection date | 1997-07-22 |
Detector | MACSCIENCE |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 117.961, 139.123, 263.264 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.020 - 2.600 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DPS D75C D78A UNPUBLISHED STRUCTURE |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (0.3) |
Refinement software | CNS (0.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.590 |
Rmerge | 0.076 | 0.291 |
Number of reflections | 75305 | |
<I/σ(I)> | 8.2 | |
Completeness [%] | 98.2 | 99.7 |
Redundancy | 3.63 | 3.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 10mM MOPS, 100mM KCl, 10% glycerol + 100mM TrisHCL, 100mM KCl, 10% glycerol, 11% PEG 8000, and 5mM DTT , pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 10mM MOPS, 100mM KCl, 10% glycerol + 100mM TrisHCL, 100mM KCl, 10% glycerol, 11% PEG 8000, and 5mM DTT , pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |