1F27
CRYSTAL STRUCTURE OF A BIOTIN-BINDING RNA PSEUDOKNOT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-09-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97186, 0.97954, 0.97969, 0.98743 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 47.590, 30.960, 62.110 |
| Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
| Resolution | 17.180 - 1.300 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23800 |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 1.770 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.380 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.048 | 0.380 |
| Total number of observations | 130824 * | |
| Number of reflections | 20852 * | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 92.0 | 66.4 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 28 * | MPD, POTASIUM HEPES, MGCL2, KCL, BIOTIN, SPERMINE, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 28 * | MPD, POTASIUM HEPES, MGCL2, KCL, BIOTIN, SPERMINE, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | POTASIUM HEPES | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | KCL | ||
| 4 | 1 | 1 | BIOTIN | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 1 | MPD | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 1 (mg/ml) | |
| 2 | 1 | drop | MPD | 3.3 (%) | |
| 3 | 1 | drop | potassium caodylate | 13.3 (mM) | |
| 4 | 1 | drop | potassium HEPES | 6.6 (mM) | |
| 5 | 1 | drop | spermine | 0.1 (mM) | |
| 6 | 1 | drop | 3.5 (mM) | ||
| 7 | 1 | drop | biotin | 3.5 (mM) | |
| 8 | 1 | reservoir | MPD | 22.5 (%) |
