1F1Z
TNSA, a catalytic component of the TN7 transposition system
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-02 |
Detector | RIGAKU RAXIS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.350, 83.680, 97.940 |
Unit cell angles | 90.00, 94.07, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.25400 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.676 |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.470 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.063 | 0.197 |
Total number of observations | 131670 * | |
Number of reflections | 23596 * | |
<I/σ(I)> | 13.2 | |
Completeness [%] | 97.0 | 80.7 |
Redundancy | 5.58 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | PEG 8000, Isopropanol, MgCl2, NaCl, Tris-HCl HEPES, Triton X100, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7-8 (mg/ml) | |
10 | 1 | buffer 1 | PEG8000 | 10 (%) | |
11 | 1 | buffer 1 | Tris-HCl | 0.1 (M) | |
12 | 1 | buffer 1 | 50 (mM) | ||
13 | 1 | reservoir | 0.1 (M) | ||
14 | 1 | reservoir | isopropanol | 10 (%) | |
15 | 1 | reservoir | buffer 1 | 90 % | |
2 | 1 | drop | HEPES | 25 (mM) | |
3 | 1 | drop | 0.5 (M) | ||
4 | 1 | drop | EDTA | 1 (mM) | |
5 | 1 | drop | dithiothreitol | 2 (mM) | |
6 | 1 | drop | Tritron X-100 | 0.01 (%(v/v)) | |
7 | 1 | drop | glycerol | 10 (%(w/v)) | |
8 | 1 | drop | isopropanol | 10 (%(v/v)) | |
9 | 1 | drop | buffer 1 |