1F1S

CRYSTAL STRUCTURE OF STREPTOCOCCUS AGALACTIAE HYALURONATE LYASE AT 2.1 ANGSTROM RESOLUTION.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1998-10-15
Detector	BRANDEIS
Spacegroup name	C 2 2 21
Unit cell lengths	51.265, 156.765, 237.429
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.191 *
Rwork	0.191
R-free	0.25300
RMSD bond length	0.005 *
RMSD bond angle	1.145 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.052 *	0.172 *
Total number of observations	484052 *
Number of reflections	54880
<I/σ(I)>	19.2
Completeness [%]	97.0	77.2 *
Redundancy	3.59	1.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	295	Jedrzejas, M.J., (2000) Acta Crystallogr., Sect.D, 56, 460. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	cacodylate	100 (mM)	pH6.0
2	1	reservoir	KSCN	30 (mM)
3	1	reservoir	PEG5000 MME	19 (%)
4	1	drop	protein	10 (mg/ml)
5	1	drop	HEPES	10 (mM)
6	1	drop	EDTA	2 (mM)	pH8.0