1F1H
CRYSTAL STRUCTURE OF GLUTAMINE SYNTHETASE FROM SALMONELLA TYPHIMURIUM WITH THALLIUM IONS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-03-31 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 231.130, 132.790, 196.780 |
Unit cell angles | 90.00, 102.44, 90.00 |
Refinement procedure
Resolution | 32.000 - 2.670 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMEN |
RMSD bond length | 0.010 |
RMSD bond angle | 25.070 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | 2.800 |
High resolution limit [Å] | 2.670 | 2.670 |
Rmerge | 0.130 | 0.430 |
Number of reflections | 130547 * | |
<I/σ(I)> | 7.8 | |
Completeness [%] | 82.0 | 55 |
Redundancy | 2 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | ADP, MPD, SPERMINE, MANGANESE CHLORIDE, IMIDAZOLE BUFFER, pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | imidazole/HCl | 15 (mM) | |
2 | 1 | 1 | 3 (mM) | ||
3 | 1 | 1 | spermine tetrahydrochloride | 3 (mM) | |
4 | 1 | 1 | MPD | 10 (%) |