1F0B

CRYSTAL STRUCTURE OF THE GREEN FLUORESCENT PROTEIN (GFP) VARIANT YFP-H148Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-10-04
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	51.661, 62.646, 66.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.500 - 2.100
R-factor	0.188
Rwork	0.188
RMSD bond length	0.020
RMSD bond angle	3.060
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.500	2.150
High resolution limit [Å]	2.070	2.070
Rmerge	0.083	0.320
Total number of observations	29297 *
Number of reflections	11419
<I/σ(I)>	10.3
Completeness [%]	82.1	81.5
Redundancy	2.56	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9 *	4 *	PEG 1550, sodium acetate, magnesium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris	20 (mM)
3	1	reservoir	PEG1550	22 (%(w/v))
4	1	reservoir	sodium acetate	100 (mM)
5	1	reservoir		90 (mM)