1F06
THREE DIMENSIONAL STRUCTURE OF THE TERNARY COMPLEX OF CORYNEBACTERIUM GLUTAMICUM DIAMINOPIMELATE DEHYDROGENASE NADPH-L-2-AMINO-6-METHYLENE-PIMELATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-07-28 |
| Detector | XENTRONICS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.547, 65.591, 84.281 |
| Unit cell angles | 90.00, 106.52, 90.00 |
Refinement procedure
| Resolution | 48.600 - 2.100 |
| R-factor | 0.178 * |
| Rwork | 0.179 |
| R-free | 0.20900 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 26.400 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 2.250 |
| High resolution limit [Å] | 2.100 | 2.110 |
| Rmerge | 0.076 | 0.224 |
| Number of reflections | 39441 * | |
| <I/σ(I)> | 8.615 | |
| Completeness [%] | 86.9 | 67 * |
| Redundancy | 2.82 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | PEG 8000, magnesium acetate, cacodylate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 14 (%) | |
| 2 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 4 | 1 | drop | protein | 24 (mg/ml) | |
| 5 | 1 | drop | HEPES | 20 (mM) | |
| 6 | 1 | drop | NADPH | 5 (mM) | |
| 7 | 1 | drop | L-2-amino-6-methylene-pimelate | 20 (mM) |
