1EZQ
CRYSTAL STRUCTURE OF HUMAN COAGULATION FACTOR XA COMPLEXED WITH RPR128515
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04-30 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.196, 71.922, 78.587 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.26700 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.061 | 0.236 |
Number of reflections | 15689 | |
<I/σ(I)> | 12.1 | |
Completeness [%] | 93.9 | 91.8 |
Redundancy | 2.7 | 2.52 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 19 * | 18-20% PEG 600, 50mM Mes-NaOH, 1mM RPR128515, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | MES-NaOH | 5 (mM) | |
3 | 1 | drop | 5 (mM) | ||
4 | 1 | drop | unit | 0.001 (mM) | |
5 | 1 | reservoir | PEG600 | 18-20 (%) | |
6 | 1 | reservoir | MES- NaOH | 50 (mM) |