1EZK
Crystal structure of recombinant tryparedoxin I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.104, 39.730, 99.173 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.28300 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 0.044 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.500 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.069 * | 0.128 |
| Number of reflections | 11980 | |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 96.5 | 81.1 |
| Redundancy | 4.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.7 * | 294 * | Kalisz, H.M., (1998) Acta Crystallogr., Sect.D, 55, 696. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 13.5 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 33 (%(w/w)) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.7 |
| 4 | 1 | reservoir | sodium acetate | 50 (mM) |
