1EZG

CRYSTAL STRUCTURE OF ANTIFREEZE PROTEIN FROM THE BEETLE, TENEBRIO MOLITOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-10
Detector	MARRESEARCH
Spacegroup name	P 65
Unit cell lengths	73.832, 73.832, 53.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	5.000 - 1.400
R-factor	0.16
Rwork	0.161
R-free	0.19960
RMSD bond length	0.011
RMSD bond angle	2.154
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.056	0.208
Number of reflections	32557
<I/σ(I)>	10.1
Completeness [%]	96.5	95.6
Redundancy	4.5	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9 *	295	ammonium sulfate, sodium citra, cobalt chloridee, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir		1.5 (M)
3	1	reservoir	glycine-NaOH	100 (mM)