1EZ4

CRYSTAL STRUCTURE OF NON-ALLOSTERIC L-LACTATE DEHYDROGENASE FROM LACTOBACILLUS PENTOSUS AT 2.3 ANGSTROM RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Collection date	1998-03-07
Detector	WEISSENBERG
Spacegroup name	P 21 21 21
Unit cell lengths	149.351, 145.544, 111.926
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.000 - 2.300
R-factor	0.205 *
Rwork	0.205
R-free	0.24100
RMSD bond length	0.016
RMSD bond angle	1.692
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.125 *	0.367
Total number of observations	1078131 *
Number of reflections	123693
<I/σ(I)>	8.3
Completeness [%]	99.3	96.7
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	25 *	ammonium sulfate, sodium acetate, NADH, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	4.6	25 *	ammonium sulfate, sodium acetate, NADH, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	acetate/NaOH	100 (mM)
2	1	reservoir	ammonium sulfate	1.2 (M)
3	1	reservoir	NADH	1 (mM)