1EZ3
CRYSTAL STRUCTURE OF THE NEURONAL T-SNARE SYNTAXIN-1A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-27 |
| Detector | OTHER |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.410, 99.560, 54.960 |
| Unit cell angles | 90.00, 97.46, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.233 |
| Rwork | 0.231 |
| R-free | 0.28640 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.748 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.046 | 0.423 |
| Total number of observations | 155471 * | |
| Number of reflections | 48323 | |
| <I/σ(I)> | 37.9 | |
| Completeness [%] | 97.4 | 78.2 |
| Redundancy | 4.53 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 23 * | PEG 1500, sodium acetate, DTT, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 10 (mM) | |
| 3 | 1 | reservoir | PEG1500 | 16 (%) | |
| 4 | 1 | reservoir | sodium acetate | 20 (mM) | pH5.0 |
| 5 | 1 | reservoir | dithiothreitol | 10 (mM) |
