1EYX

CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN AT 2.2 ANGSTROMS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-08-31
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	187.268, 187.268, 59.109
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	999.000 - 2.250
R-factor	0.192
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lia
RMSD bond length	0.004
RMSD bond angle	0.012
Data reduction software	XDS (V. 1998)
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.550	2.280
High resolution limit [Å]	2.160	2.160
Rmerge	0.101	0.039
Total number of observations	123143 *
Number of reflections	38751
<I/σ(I)>	7.3	2.8
Completeness [%]	98.7	89.4
Redundancy	3.1	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294 *	200 MM HEPES, 500 MM SODIUM CHLORIDE, 50 MM POTASIUM CHLORIDE, 15 MM SODIUM AZIDE, 16% AMMONIUM SULPHATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12-15 (mg/ml)
2	1	reservoir	ammonium sulfate	16-18 (%sat)
3	1	reservoir	HEPES	200 (mM)
4	1	reservoir	sodium chloride	500 (mM)
5	1	reservoir	sodium azide	15 (mM)