1EYI

FRUCTOSE-1,6-BISPHOSPHATASE COMPLEX WITH MAGNESIUM, FRUCTOSE-6-PHOSPHATE AND PHOSPHATE (R-STATE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	120
Detector technology	AREA DETECTOR
Collection date	1998-08-01
Detector	SIEMENS
Spacegroup name	I 2 2 2
Unit cell lengths	52.120, 82.510, 165.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 2.320
Rwork	0.190
R-free	0.24200
Starting model (for MR)	1cnq
RMSD bond length	0.005
RMSD bond angle	22.600 *
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.460
High resolution limit [Å]	2.320	2.320
Rmerge	0.640	0.239
Total number of observations	72680 *
Number of reflections	30028
<I/σ(I)>	15.6
Completeness [%]	96.1	75.5
Redundancy	4.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	37 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		10 (mM)
3	1	drop		5 (mM)	or 5 mM MgCl2
4	1	drop	F6P	(mM)
5	1	reservoir	Tris-malonate	(mM)
6	1	reservoir	PEG3350	(%(w/v))