1EYG

Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	103
Detector technology	CCD
Collection date	1998-03-25
Spacegroup name	C 1 2 1
Unit cell lengths	98.688, 71.081, 79.160
Unit cell angles	90.00, 91.93, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.256
Rwork	0.256
R-free	0.29800
RMSD bond length	0.017
RMSD bond angle	2.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.035 *	0.080 *
Total number of observations	44241 *
Number of reflections	13271 *
Completeness [%]	97.6 *	96.7 *
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	291	PEG 4000,PEG 200, Hepes, pH 7.5, vapor diffusion, macro seeding, temperature 291K, VAPOR DIFFUSION

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Hepes
2	1	1	PEG 200
3	1	1	PEG 4000
4	1	2	PEG 200
5	1	2	PEG 4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)
2	1	reservoir	PEG200	30 (%(v/v))
3	1	reservoir	PEG4000	20 (%(v/v))