1EXN
T5 5'-EXONUCLEASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BL19 |
| Synchrotron site | ESRF |
| Beamline | BL19 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 |
| Unit cell lengths | 77.760, 77.760, 134.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.33900 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.037 |
| Total number of observations | 67552 * |
| Number of reflections | 26463 |
| Completeness [%] | 95.2 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | Ceska, T.A., (1993) J.Mol.Biol., 233, 179. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 35 (%(w/v)) | |
| 4 | 1 | drop | potassium phosphate | 25 (mM) | pH7.5 |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | dithiothreitol | 1 (mM) | |
| 7 | 1 | drop | 100 (mM) | ||
| 8 | 1 | drop | glycerol | 5 (%(v/v)) |
