1EXM

CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS ELONGATION FACTOR TU (EF-TU) IN COMPLEX WITH THE GTP ANALOGUE GPPNHP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-21
Temperature [K]	298
Detector technology	DIFFRACTOMETER
Detector	ENRAF-NONIUS FAST
Spacegroup name	C 1 2 1
Unit cell lengths	150.500, 99.500, 40.200
Unit cell angles	90.00, 95.30, 90.00

Refinement procedure

Resolution	14.780 - 1.700
R-factor	0.209
Rwork	0.209
R-free	0.24300
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	MADNESS
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR
Refinement software	CNS (1.0)

Data quality characteristics

	Outer shell
Low resolution limit [Å]	1.810
High resolution limit [Å]	1.700
Completeness [%]	43.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20mM Sodium cacodylate, 5mM Magnesium sulfate, 38-44% Ammonium sulfate, 7-10 mg/mL EF-Tu, molar ratio EF-Tu:GppNHp = 1:5, pH 7.0, VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	7	293	20mM Sodium cacodylate, 5mM Magnesium sulfate, 38-44% Ammonium sulfate, 7-10 mg/mL EF-Tu, molar ratio EF-Tu:GppNHp = 1:5, pH 7.0, VAPOR DIFFUSION, HANGING DROP