1EXD
CRYSTAL STRUCTURE OF A TIGHT-BINDING GLUTAMINE TRNA BOUND TO GLUTAMINE AMINOACYL TRNA SYNTHETASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-01-17 |
| Detector | OTHER |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 236.940, 93.980, 113.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.700 |
| Rwork | 0.216 |
| R-free | 0.28900 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | X-PLOR (98.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 | 0.407 |
| Number of reflections | 34389 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 97.7 | 93.9 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | (NH4)2SO4, Hepes, b-mercaptoethanol, MgSO4 at 298 K, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | b-mercaptoethanol | ||
| 2 | 1 | 1 | Hepes | ||
| 3 | 1 | 1 | MgSO4 | ||
| 4 | 1 | 1 | (NH4)2SO4 | ||
| 5 | 1 | 2 | MgSO4 | ||
| 6 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | magnesium chloride | 5 (mM) | |
| 3 | 1 | drop | GlnRS | 4.5 (mg/ml) | |
| 4 | 1 | drop | PIPES | 7.5 (mM) | |
| 5 | 1 | drop | beta-mercaptoethanol | 2.5 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
| 7 | 1 | reservoir | PIPES | 70 (mM) | |
| 8 | 1 | reservoir | 20 (mM) |
