1EXB

STRUCTURE OF THE CYTOPLASMIC BETA SUBUNIT-T1 ASSEMBLY OF VOLTAGE-DEPENDENT K CHANNELS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-22
Detector	BRANDEIS - B4
Spacegroup name	P 4 21 2
Unit cell lengths	100.725, 100.725, 110.901
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.100
R-factor	0.216
Rwork	0.215
R-free	0.22700
RMSD bond length	0.007
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.080	0.300
Number of reflections	33500
<I/σ(I)>	17.8
Completeness [%]	98.8	92.9
Redundancy	11.8	9689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.6	277	Peg 4000, glycine, pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	glycine	50 (mM)
3	1	reservoir	PEG400	6 (%)