1EX6
CRYSTAL STRUCTURE OF UNLIGANDED FORM OF GUANYLATE KINASE FROM YEAST
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-03-24 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 31 |
| Unit cell lengths | 51.559, 51.559, 119.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.164 * |
| Rwork | 0.175 |
| R-free | 0.25900 |
| Structure solution method | HGMAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.017 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.543 |
| Total number of observations | 37285 * | |
| Number of reflections | 15759 | |
| <I/σ(I)> | 9.9917 | 1.5845 |
| Completeness [%] | 99.7 | 97.9 |
| Redundancy | 2.37 | 2.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 100 * | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
| 4 | 1 | reservoir | sodium acetate | 0.3 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) |
