1EX3
CRYSTAL STRUCTURE OF BOVINE CHYMOTRYPSINOGEN A (TETRAGONAL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-17 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 114.900, 114.900, 52.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.900 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.092 | 0.268 |
| Number of reflections | 7446 | |
| <I/σ(I)> | 17.63 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.16 | 5.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 5 | 25 * | 0.1 M NaCl, 10 mM Na citrate, 0.05% Na azide, 1 mM phenylmethanesulphonyl-fluoride, pH 5.0, Centrifugal crystallization, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (g/L) | |
| 2 | 1 | 1 | 0.1 (M) | ||
| 3 | 1 | 1 | sodium citrate | 10 (mM) | |
| 4 | 1 | 1 | sodium azide | 0.05 (%) | |
| 5 | 1 | 1 | PMSF | 1 (mM) |
