1EWY

ANABAENA PCC7119 FERREDOXIN:FERREDOXIN-NADP+-REDUCTASE COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-11-06
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	63.730, 63.720, 158.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.380
R-factor	0.219 *
Rwork	0.219
R-free	0.29200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1QUE FOR FERREDOXIN-NADP+REDUCTASE
RMSD bond length	0.022 *
RMSD bond angle	0.022 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.500
High resolution limit [Å]	2.380	2.280
Rmerge	0.046	0.077
Total number of observations	175226 *
Number of reflections	25838
<I/σ(I)>	7.9	4.2
Completeness [%]	97.2	87.7
Redundancy	4.3	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	293 *	20-24% PEG 6000,10 mM MES pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	oxidized FNR	0.7 (mM)
2	1	drop	oxidized Fd	0.5 (mM)
3	1	drop	Tris-HCl	0.1 (M)	pH8.0
4	1	drop	beta-octylglucoside	5 (%)
5	1	reservoir	MES	0.1 (M)	pH5.5
6	1	reservoir	PEG6000	18-21 (%(w/v))
7	1	reservoir	sodium azide	0.1 (%(w/v))