1EVZ
CRYSTAL STRUCTURE OF LEISHMANIA MEXICANA GLYCEROL-3-PHOSPHATE DEHYDROGENASE IN COMPLEX WITH NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 125 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 69.850, 69.850, 211.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.25500 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.230 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.066 | 0.183 |
| Total number of observations | 93667 * | |
| Number of reflections | 13000 | |
| <I/σ(I)> | 27.63 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 * | 298 | Triethanol amine pH 7.25 Sodium Citrate 0.9M, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | TEA | 20 (mM) | |
| 3 | 1 | drop | NAD | 4 (mM) | |
| 4 | 1 | drop | dithiothreitol | 4 (mM) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | PMSF | 0.2 (mM) | |
| 7 | 1 | reservoir | sodium citrate | 0.9 (M) |
