1EVT

CRYSTAL STRUCTURE OF FGF1 IN COMPLEX WITH THE EXTRACELLULAR LIGAND BINDING DOMAIN OF FGF RECEPTOR 1 (FGFR1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1999-03-25
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1
Unit cell lengths	62.553, 64.061, 64.139
Unit cell angles	93.40, 111.17, 97.18

Refinement procedure

Resolution	25.000 - 2.800
R-factor	0.249
Rwork	0.249
R-free	0.30000
RMSD bond length	0.009
RMSD bond angle	0.960 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.083	0.226
Total number of observations	49288 *
Number of reflections	22330 *
<I/σ(I)>	8.6
Completeness [%]	97.9	90.5
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	20 *	PEG 4000, Isopropanol, HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mg/ml)
2	1	drop	Tris-HCl	0.1 (M)
3	1	reservoir	PEG4000	30 (%)
4	1	reservoir		0.2 (M)
5	1	reservoir	Tris-HCl	0.1 (M)