1EVI
THREE-DIMENSIONAL STRUCTURE OF THE PURPLE INTERMEDIATE OF PORCINE KIDNEY D-AMINO ACID OXIDASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-06-04 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 107.730, 91.370, 71.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.239 |
Rwork | 0.232 |
R-free | 0.29800 |
RMSD bond length | 0.006 |
RMSD bond angle | 24.210 * |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.067 | 0.151 |
Total number of observations | 86285 * | |
Number of reflections | 23734 | |
<I/σ(I)> | 18 | |
Completeness [%] | 96.5 | 95.9 |
Redundancy | 3.56 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 20 * | Setoyama, C., (1996) J. Biochem., 1114. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 3.5 (mg/ml) | |
2 | 1 | drop | sodium acetate | 120 (mM) | |
3 | 1 | drop | sodium citrate | 60 (mM) | |
4 | 1 | drop | sodium benzoate | 0.2 (mM) | |
5 | 1 | drop | PEG4000 | 5 (%) | |
6 | 1 | reservoir | sodium acetate | 200 (mM) | |
7 | 1 | reservoir | sodium citrate | 100 (mM) | |
8 | 1 | reservoir | PEG4000 | 30 (%) |