1EUS

SIALIDASE COMPLEXED WITH 2-DEOXY-2,3-DEHYDRO-N-ACETYLNEURAMINIC ACID

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	48.000, 82.860, 84.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.159
Rwork	0.159
RMSD bond length	0.020
RMSD bond angle	2.740
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.063	0.112 *
Total number of observations	93040 *
Number of reflections	20366
Completeness [%]	83.0	67.4 *
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			Taylor, G., (1992) J.Mol.Biol., 225, 1135. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	8 (%)