1EUO

Crystal structure of nitrophorin 2 (prolixin-S)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	298
Detector technology	DIFFRACTOMETER
Collection date	1998-10-30
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 21 21 2
Unit cell lengths	40.340, 127.960, 33.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.200 - 2.000
R-factor	0.19 *
Rwork	0.195
R-free	0.24000 *
RMSD bond length	0.006 *
RMSD bond angle	1.270
Data reduction software	MADNESS
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.200	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.106	0.348 *
Total number of observations	49410 *
Number of reflections	12186
<I/σ(I)>	4.5
Completeness [%]	96.9	90.3
Redundancy	4.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	298	ammonium phosphate, Tris-HCl, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium phosphate	2.8 (M)
2	1	reservoir	Tris-HCl	0.1 (M)