1ETZ

THE THREE-DIMENSIONAL STRUCTURE OF AN ANTI-SWEETENER FAB, NC10.14, SHOWS THE EXTENT OF STRUCTURAL DIVERSITY IN ANTIGEN RECOGNITION BY IMMUNOGLOBULINS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1993-07-09
Detector	SIEMENS
Spacegroup name	P 1
Unit cell lengths	55.900, 66.300, 75.200
Unit cell angles	106.70, 107.60, 97.30

Refinement procedure

Resolution	10.000 - 2.600
R-factor	0.211
Rwork	0.198
R-free	0.29400
RMSD bond length	0.008
RMSD bond angle	28.000 *
Data reduction software	X-GEN
Data scaling software	X-GEN
Phasing software	MERLOT
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.073	0.125
Total number of observations	101821 *
Number of reflections	26144
<I/σ(I)>	16.2
Completeness [%]	90.3	66.8
Redundancy	3.9	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7	290	NC10.14 Fab with excess molarity NC174, 10%(w/v) PEG 8000, 0.05M NaCl, 0.05%(w/v) Sodium Azide, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	35 (mg/ml)
2	1	1		0.05 (M)
3	1	1	sodium azide	0.05 (%)
4	1	1	PEG8000	33 (%(w/w))