1ES8
Crystal structure of free BglII
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-03-01 |
Detector | BRANDEIS - B4 |
Wavelength(s) | 0.9793, 0.9789, 0.9689 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 79.150, 79.150, 66.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
Rwork | 0.214 |
R-free | 0.25800 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.270 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.057 * | 0.138 * |
Total number of observations | 33794 * | |
Number of reflections | 10979 | |
<I/σ(I)> | 14.4 | |
Completeness [%] | 99.4 | 99.1 |
Redundancy | 3.1 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 4 * | drop consists of equal amounts of protein and precipitant solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | glycerol | 10 (%(v/v)) | |
5 | 1 | drop | dithiothreitol | 1 (mM) | |
6 | 1 | reservoir | isopropanol | 30 (%(v/v)) | precipitant |
7 | 1 | reservoir | sodium acetate | 0.2 (M) | precipitant |
8 | 1 | reservoir | Bis-tris | 100 (mM) | precipitant |