1ES1
CRYSTAL STRUCTURE OF VAL61HIS MUTANT OF TRYPSIN-SOLUBILIZED FRAGMENT OF CYTOCHROME B5
Replaces: 1QDXExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.757, 40.433, 39.306 |
| Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.100 |
| Rwork | 0.192 |
| R-free | 0.24400 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.150 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.232 |
| Number of reflections | 5579 | |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 91.7 | 79.5 |
| Redundancy | 1.93 | 1.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | phosphate buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | phosphate | 3.2 (M) | pH8.0 |
| 2 | 1 | drop | protein | 20 (mg/ml) |
