1ERU

HUMAN THIOREDOXIN (OXIDIZED FORM)

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1995-09-22
Detector	ENRAF-NONIUS FAST
Spacegroup name	C 1 2 1
Unit cell lengths	67.830, 26.350, 51.790
Unit cell angles	90.00, 94.94, 90.00

Refinement procedure

Resolution	15.000 - 2.100
R-factor	0.22
Rwork	0.220
R-free	0.33000
RMSD bond length	0.016
RMSD bond angle	2.800
Data reduction software	MADNES
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.100 *
Rmerge	0.049 *
Total number of observations	8894 *
Number of reflections	4440
Completeness [%]	80.0
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	3.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium acetate	10 (mM)
2	1	drop	MPD	25 (%(v/v))
3	1	drop	DTT	5 (mM)
4	1	reservoir	MPD	50 (%)