1EQ6
1.9 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF THE SACCHAROMYCES CEREVISIAE RAN-BINDING PROTEIN MOG1P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.672, 51.963, 112.234 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.2185 |
| Rwork | 0.217 |
| R-free | 0.24580 |
| Structure solution method | MAD PLUS SIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 25.320 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.039 | 0.146 * |
| Number of reflections | 18625 | |
| <I/σ(I)> | 11.5 | 4.9 |
| Completeness [%] | 98.3 | 98.7 * |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 * | 291 | Baker, R.P.,(2000) Acta Crystallog. Sect., D56, 229. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 2 | 1 | reservoir | sodium citrate | 1 (M) | |
| 3 | 1 | reservoir | PEG4000 | 30 (%) | |
| 4 | 1 | drop | protein | 10-12 (mg/ml) |
