1EP9
HUMAN ORNITHINE TRANSCARBAMYLASE: CRYSTALLOGRAPHIC INSIGHTS INTO SUBSTRATE RECOGNITION AND CONFORMATIONAL CHANGE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-08-27 |
| Detector | SIEMENS |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 125.600, 125.600, 125.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| Rwork | 0.184 |
| R-free | 0.22300 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.600 * |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.111 | 0.402 |
| Total number of observations | 333653 * | |
| Number of reflections | 26061 | |
| <I/σ(I)> | 23.3 | |
| Completeness [%] | 98.4 | 93.2 |
| Redundancy | 12.8 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 18 * | 20 MM TRISAC, 2MM EDTA, 20MM KCL, 4MM PALO, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | PALO | 4 (mM) | |
| 3 | 1 | drop | Tris acetate | 20 (mM) | |
| 4 | 1 | drop | EDTA | 2 (mM) | |
| 5 | 1 | drop | 20 (mM) | ||
| 6 | 1 | reservoir | PEG400 | 2 (%) | |
| 7 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 8 | 1 | reservoir | sodium HEPES | 0.1 (M) |
