1EOU
CRYSTAL STRUCTURE OF HUMAN CARBONIC ANHYDRASE II COMPLEXED WITH AN ANTICONVULSANT SUGAR SULFAMATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-23 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.275, 41.390, 72.509 |
| Unit cell angles | 90.00, 104.11, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 2.100 |
| R-factor | 0.018 * |
| Rwork | 0.176 |
| R-free | 0.02300 * |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.450 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.060 |
| High resolution limit [Å] | 2.000 | 1.800 * |
| Rmerge | 0.070 | 0.110 |
| Total number of observations | 169841 * | |
| Number of reflections | 16971 * | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 92.3 | 77.2 * |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 4 * | TRIS.HCl, smmonium sulfate, dithiothreitol, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 4 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.8 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 50 (mM) | pH8.8 |
