1EO5
Bacillus circulans strain 251 cyclodextrin glycosyltransferase in complex with maltoheptaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 116.700, 109.700, 67.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.167 * |
| Rwork | 0.167 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cxi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.652 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.900 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.111 | 0.414 |
| Number of reflections | 54766 | |
| <I/σ(I)> | 7.72 | 2.9 |
| Completeness [%] | 92.1 | 88.1 |
| Redundancy | 3.48 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.1 * | Lawson, C.L., (1994) J. Mol. Biol., 236, 590. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 60 (%(v/v)) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 3 | 1 | reservoir | maltose | 5 (%(w/v)) |
