1ENV
ATOMIC STRUCTURE OF THE ECTODOMAIN FROM HIV-1 GP41
Experimental procedure
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-13 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1997-02 |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 52.350, 52.350, 414.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.600 |
R-factor | 0.244 |
Rwork | 0.244 |
R-free | 0.27700 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.590 |
Rmerge | 0.071 | 0.179 |
Number of reflections | 8130 | |
<I/σ(I)> | 10.2 | 4.5 |
Completeness [%] | 90.5 | 91.4 |
Redundancy | 7.45 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | 20 MM HEPES PH 8.0 1.1 M AMMONIUM SULFATE 12% ETHYLENE GLYCOL SPACE GROUP R32 IN HEXAGONAL SETTING |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15-20 (mg/ml) | |
2 | 1 | drop | HEPES | 20 (mg/ml) | |
3 | 1 | drop | 75 (mM) | ||
4 | 1 | reservoir | HEPES | 100 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 1.1 (M) | |
6 | 1 | reservoir | ethylene glycol | 12 (%) |