1EMV

CRYSTAL STRUCTURE OF COLICIN E9 DNASE DOMAIN WITH ITS COGNATE IMMUNITY PROTEIN IM9 (1.7 ANGSTROMS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-19
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	43.870, 52.560, 87.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.219 *
Rwork	0.219
R-free	0.26000
RMSD bond length	0.010
RMSD bond angle	1.538
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.026	0.071
Total number of observations	415140 *
Number of reflections	20871
<I/σ(I)>	47.1
Completeness [%]	89.9	73.4
Redundancy	19.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.3	277	used seeding, Kuhlmann, U.C., (1999) Acta. Crystallog. sect. D55, 256. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	32 (%(w/v))
2	1	reservoir	sodium acetate	0.1 (M)