1EMT

FAB ANTIBODY FRAGMENT OF AN C60 ANTIFULLERENE ANTIBODY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	57.900, 65.745, 65.562
Unit cell angles	90.00, 112.45, 90.00

Refinement procedure

Resolution	60.000 - 2.250
R-factor	0.18 *
Rwork	0.180
R-free	0.24000
RMSD bond length	0.018 *
RMSD bond angle	2.106 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
High resolution limit [Å]	2.250
Rmerge	0.090
Number of reflections	19413
Completeness [%]	90.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *		10% PEG 8000, 0.1 M citrate, Fab at 7 mg/ml concentration, pH 5.0, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Fab	7 (mg/ml)
2	1	drop	Tris	10 (mM)
3	1	reservoir	PEG8000	10 (%)
4	1	reservoir	citrate	0.1 (M)