1EMB

GREEN FLUORESCENT PROTEIN (GFP) FROM AEQUOREA VICTORIA, GLN 80 REPLACED WITH ARG

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1995-09
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	51.900, 63.200, 68.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.130
R-factor	0.196 *
Rwork	0.196
R-free	0.25400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema
RMSD bond length	0.011
RMSD bond angle	1.534
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.200
High resolution limit [Å]	2.130	2.130
Rmerge	0.061 *	0.186 *
Total number of observations	60182 *
Number of reflections	13001
<I/σ(I)>	7.1	3.9
Completeness [%]	91.3	54.5
Redundancy	4.8	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *		PROTEIN WAS CRYSTALLIZED FROM 50 MM KH2PO4 AND 20 % (W/V) PEG 8000, PH 3.8.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	GFP	11 (mg/ml)
2	1	drop	Tris	7 (mM)
3	1	drop		0.01 (%)
4	1	drop		0.01 (M)
5	1	drop	PEG8000	0.03 (%(w/v))
6	1	reservoir		0.05 (M)
7	1	reservoir	PEG8000	20 (%(w/v))