1EM2

Star-related lipid transport domain of MLN64

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	95
Detector technology	CCD
Collection date	1999-11-16
Detector	OTHER
Spacegroup name	P 31 2 1
Unit cell lengths	83.417, 83.417, 81.946
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.200
R-factor	0.207 *
Rwork	0.207
R-free	0.26400
RMSD bond length	0.017 *
RMSD bond angle	1.850 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.200
Rmerge	0.065
Total number of observations	136560 *
Number of reflections	16818 *
Completeness [%]	98.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	24 *	K/Na tartrare, lithium sulfate, CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	CHES	100 (mM)	pH9.5
2	1	reservoir	sodium potassium tartrate	950 (mM)
3	1	reservoir		200 (mM)