1ELM
CADMIUM-SUBSTITUTED BOVINE PACREATIC CARBOXYPEPTIDASE A (ALFA-FORM) AT PH 5.5 AND 2 MM CHLORIDE IN MONOCLINIC CRYSTAL FORM.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09-29 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.163, 60.244, 51.746 |
| Unit cell angles | 90.00, 97.41, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.161 |
| Rwork | 0.161 |
| R-free | 0.18630 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cpa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.260 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.010 |
| Rmerge | 0.068 | 0.165 |
| Number of reflections | 107945 * | |
| <I/σ(I)> | 19.6 | 7.6 |
| Completeness [%] | 96.2 | 82.2 |
| Redundancy | 4.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 * | 293 | Lithium chloride, Tris-HNO3, Cadmium chloride, pH 5.50, MICRODIALYSIS, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 1.0 (M) | ||
| 2 | 1 | 1 | Tris-HNO3 | 20 (mM) | pH7.5 |
| 3 | 1 | 1 | protein | 20 (mg/ml) | |
| 4 | 1 | 2 | 0.35 (M) | ||
| 5 | 1 | 2 | Tris-HNO3 | 20 (mM) | pH7.5 |
