1EKP

CRYSTAL STRUCTURE OF HUMAN BRANCHED CHAIN AMINO ACID AMINOTRANSFERASE (MITOCHONDRIAL) COMPLEXED WITH PYRIDOXAL-5'-PHOSPHATE AT 2.5 ANGSTROMS (MONOCLINIC FORM).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-03-22
Detector	RIGAKU RAXIS II
Spacegroup name	P 1 21 1
Unit cell lengths	59.630, 111.640, 59.900
Unit cell angles	90.00, 95.89, 90.00

Refinement procedure

Resolution	40.650 - 2.500
R-factor	0.166
Rwork	0.155
R-free	0.22000 *
Starting model (for MR)	E. COLI BRANCHED CHAIN AMINO ACID AMINOTRANSFERASE
RMSD bond length	0.008 *
RMSD bond angle	1.332 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.650	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.063	0.203
Number of reflections	21222
<I/σ(I)>	13.1
Completeness [%]	78.6 *	66.8
Redundancy	2.632	1.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG1500, DTT, HEPES; NA OR K PHOSPHATE, DTT, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.5 (mg/ml)
2	1	drop	HEPES	50 (mm)
3	1	drop	dithiothreitol	20 (mM)
4	1	drop	EDTA	50 (mM)
5	1	reservoir	sodium phosphate	1.6 (M)	or potassium phosphate
6	1	reservoir	dithiothreitol	20 (mM)
7	1	reservoir	HEPES	0.1 (M)