1EK6
STRUCTURE OF HUMAN UDP-GALACTOSE 4-EPIMERASE COMPLEXED WITH NADH AND UDP-GLUCOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-21 |
| Detector | SBC-2 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.700, 86.900, 95.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.19800 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.040 |
| Data reduction software | MADNESS |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.286 |
| Number of reflections | 96156 | 8401 * |
| <I/σ(I)> | 29.9 | |
| Completeness [%] | 96.1 | 84.7 |
| Redundancy | 5.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6 | 273 | PEG 3400, magnesium chloride, MES, NADH, UDP-glucose, sodium chloride, pH 6.0, BATCH, temperature 273.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | MES | 100 (mM) | pH6.0 |
| 2 | 1 | 2 | PEG3400 | 8.5 (%) | |
| 3 | 1 | 2 | 75 (mM) |
