1EJ4

COCRYSTAL STRUCTURE OF EIF4E/4E-BP1 PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-09-15
Detector	RIGAKU RAXIS IIC
Spacegroup name	C 1 2 1
Unit cell lengths	81.900, 45.200, 62.100
Unit cell angles	90.00, 101.80, 90.00

Refinement procedure

Resolution	25.000 - 2.250
Rwork	0.219
R-free	0.26700
RMSD bond length	0.009 *
RMSD bond angle	1.550 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.052	0.103
<I/σ(I)>	24.9
Completeness [%]	97.4	84.8
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	24 (%)
2	1	reservoir	isopropanol	10 (%)
3	1	reservoir	HEPES	0.1 (M)